Abstract. Dissecting immune responses by next generation sequencing methods at single cell resolution provides novel insights into cell states, including antigen receptor diversity, cell-cell interactions in tissues, and how cellular phenotypes change in the context of adaptation from lymphoid to non-lymphoid tissues.
Here I will describe our recently developed computational methods for single cell trajectory and bifurcation inference of transcriptomes and antigen receptor sequences. I will also present applications of these methods to reconstruct tissue adaptation trajectories of regulatory T cells in mouse and man, and application to analysis of immune cells within barrier tissues such as lung and decidua.
Short Bio. Sarah Teichmann is Head of Cellular Genetics at the Wellcome Trust Sanger Institute. Her work focuses on deciphering the immune system with genomics and bioinformatics approaches. She co-chairs the international Human Cell Atlas Consortium together with Aviv Regev (Broad Institute)
Sarah did her PhD at the MRC Laboratory of Molecular Biology, Cambridge, UK with Dr Cyrus Chothia, FRS and was a Beit Memorial Fellow at University College London with Professor Dame Janet Thornton, FMedSci, FRS. She started her group at the MRC Laboratory of Molecular Biology in 2001. In 2013, she moved to the Wellcome Trust Genome Campus in Hinxton, Cambridge, jointly with the EMBL-European Bioinformatics Institute and the Sanger Institute. In January 2016 she became Head of the Cellular Genetics Programme at the Sanger Institute. She is also a Director of research in the Cavendish Laboratory, University of Cambridge and Senior Research Fellow at Churchill College.
Sarah is an EMBO member and a Fellow of the Academy of Medical Sciences, and her work has been recognized by a number of UK and international prizes, including the Lister Prize, Biochemical Society Colworth Medal, Royal Society Crick Lecture and EMBO Gold Medal.